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Nitrocellulose slide : ウィキペディア英語版 | Nitrocellulose slide A nitrocellulose slide (or nitrocellulose film slide) is a glass microscope slide that is coated with nitrocellulose that is used to bind biological material, often protein, for colorimetric and fluorescence detection assays. For this purpose, a nitrocellulose slide is generally considered to be superior to glass, because it binds a great deal more protein, and protects the tertiary structure of the protein (and other biological material, i.e.: cells). Typically, nitrocellulose slides have a thin, opaque film of nitrocellulose on a standard 25mm × 75 mm glass microscope slide. The film is extremely sensitive to contact, and to foreign material; contact causes deformation and deposition of material, especially liquids. A nitrocellulose slide is different from a nitrocellulose membrane, which usually filters protein from solution (i.e.: physician's office pregnancy tests), but that it serves a similar goal: to detect the presence and/or concentration level of certain biological material. ==Microarrays== Nitrocellulose slides are used mainly in proteomics to do protein microarrays with automated systems that print the slides and record results. Microarrays of cell analytes, arrays of cell lysate, antibody microarrays, tissue printing, immunoarrays, etc. are also possible with the slide.
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